Molecular characteristics of HBV infection among blood donors tested HBsAg reactive in a single ELISA test in southern China
نویسندگان
چکیده
Abstract Background Hepatitis B virus (HBV) infection is a major concern for blood safety in high-prevalence HBV countries such as China. In Shenzhen, dual hepatitis surface antigen (HBsAg) enzyme-linked immunosorbent assays (ELISAs) have been adopted parallel with nucleic acid testing (NAT) donors over decade. A small proportion of test reactive (R) HBsAg but negative through routine NAT, which can lead to an extremely low viral load. Objectives We aimed investigate and analyze the molecular characteristics among that tested R single ELISA test. Methods Blood donations were evaluated this study if confirmed one two kits. Samples non-reactive (NR) results by NAT collected chemiluminescent microparticle immunoassay (CLIA) neutralization The level was further assessed electrochemiluminescence (ECLIA). basic core promoter (BCP) pre-core (PC) S regions amplified nested PCR. Quantitative real-time PCR (qPCR) load determination individual donation (ID)-NAT simultaneously. CLIA, ECLIA, PCR, qPCR, ID-NAT. Results Of 100,252 donations, 38 41 identified Wantai DiaSorin kits, respectively. Seventy-nine (0.077%, 79/100,252) samples R-NR NR enrolled study. these, 17 (21.5%,17/79) HBsAg-positive. 14 genotyped cases, 78.6% (11/14) genotype B, C D observed sample, Mutations found gene, including Y100C, Y103I, G145R, L175S, affect detection HBsAg. high-frequency mutation, T1719G (93.3%), detected BCP/PC region, reduced replication. Conclusion number infection, acids most methods. It necessary employ more sensitive specific donors.
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ژورنال
عنوان ژورنال: BMC Infectious Diseases
سال: 2021
ISSN: ['1471-2334']
DOI: https://doi.org/10.1186/s12879-020-05747-4